Article Type
Original Study
Abstract
Background: Carbapenems are the drugs of choice for serious hospital-acquired infections and for patients in intensive care unit affected by multidrug-resistant microorganisms, particularly Acinetobacter baumannii. Carbapenem-resistant Acinetobacter baumannii (CRAB) poses a significant threat to public health. Aim: The study aims to focus the distribution of carbapenemase-encoding genes belonging to classes A and B (blaGES, blaKPC, and blaNDM) among CRAB isolated from various clinical specimens. Methods: A total of 55 bacterial isolates were collected from different clinical specimens and underwent identification using Chrom agar and the Vitek 2 compact (ID) system. The checkerboard technique, modified Hodge test, and Vitek 2 compact (AST) system were employed to detect CRAB. PCR techniques were used to identify the presence of blaGES, blaKPC, and blaNDM. Results: Of the 55 isolates, 43 (78.1%) were identified as A. baumannii, with 39 (90.6%) of these being CRAB. All CRAB isolates could produce carbapenemase. The percentages of CRAB isolates possessing blaGES, blaKPC, and blaNDM were 28.2%, 15.4%, and 48.7%, respectively. In contrast, 25%a percentage of non-CRAB isolates possessing blaKPC and blaNDM had 25% of each. Conclusion: A high prevalence of CRAB capable of producing carbapenemase and harboring blaGES, blaKPC, and blaNDM genes belonging to classes A and B was observed among various infections in Al Najaf hospitals.
Keywords
Acinetobacter baumannii, carbapenem resistance, carbapenemase, PCR
Recommended Citation
Rajab, Ghufran and Kadhum, Ahlam
(2025)
"Distribution of resistance genes among carbapenem-resistant Acinetobacter baumannii isolated from different clinical specimens,"
Iraqi National Journal of Medicine: Vol. 7
:
Iss.
1
, Article 17.
Available at:
https://doi.org/10.37319/iqnjm.7.1.15
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